CUT PLANT STRESS PHYSIOLOGY GROUP
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Standard protocols used routinely in our laboratory:


 ROS/RNS/RSS
  • Nitric oxide quantification
  • Hydrogen peroxide quantification
  • Hydrogen sulfide quantification
  
 Nucleic Acids
  • CTAB genomic DNA extraction
  • RNA extraction from Arabidopsis seeds and silliques
  • LiCl RNA extraction from strawberry leaves and roots
  • RNA extraction from kiwifruit
  • Trizol RNA extraction from citrus leaves and roots
  • RNA precipitation
  
 Cellular Damage
  • Lipid peroxidation (TBARS assay)
  • Pigment quantification (DMSO)
 
 Metabolites
  • Proline quantification
  • Polyamine HPLC analysis (benzoylation)

  Enzyme Activity Assays
  • Catalase (CAT)
  • Superoxide dismutase (SOD)
  • Nitrate reductase (NR)
  • P5CS (proline biosynthesis)

 Antioxidant Activity Assays
  • DPPH
  • FRAP
  • TEAC
  • Phosphomolybdate

 Redox State
  • Ascorbate
  • Glutathione
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  • Home
  • Research
    • Ongoing Research Projects
    • Completed Research Projects
  • People
    • Group Members
    • Collaborators
  • Publications
    • Journals
    • Book Chapters
    • Conference Proceedings
    • Edited Books
    • Edited Journal Special Issues
    • Popular Media
    • Patents
  • Facilities
  • Protocols
  • Teaching
  • Contact us
  • YieldShield