Standard protocols used routinely in our laboratory:


 ROS/RNS/RSS

• Nitric oxide quantification
• Hydrogen peroxide quantification
• Hydrogen sulfide quantification

  
 Nucleic Acids

• CTAB genomic DNA extraction
• RNA extraction from Arabidopsis seeds and silliques
• LiCl RNA extraction from strawberry leaves and roots
• RNA extraction from kiwifruit
• Trizol RNA extraction from citrus leaves and roots
• RNA precipitation

  
 Cellular Damage

• Lipid peroxidation (TBARS assay)
• Pigment quantification (DMSO)

 
 Metabolites

• Proline quantification

• Polyamine HPLC analysis (benzoylation)

  Enzyme Activity Assays

• Catalase (CAT)

• Superoxide dismutase (SOD)

• Nitrate reductase (NR)
• P5CS (proline biosynthesis)

 Antioxidant Activity Assays

• DPPH
• FRAP
• TEAC
• Phosphomolybdate

 Redox State

• Ascorbate
• Glutathione